Method for producing the serum that can be stored to fight herpes virus in puppies:
1. First you will need a donor. Use an 8-12 month old mixed breed dog 60-100 lbs, young and healthy. 2. Do full cbc and fecal to eliminate all possible health problems. Do not use breeds or individuals known to have immune deficiency problems. 3. Make up Newcastle virus vaccine 1000 dose vial. (Use only 10 cc of diluent. Discard balance.) La Sota strain. This virus is your cell immunity. 4. Inject 2-3cc of Newcastle virus I.V. (shock may occur. Treat with I.V fluids accordingly) 5. Induction of vaccine may only be done once on any dog. The second time around only antibodies to disease is produced. 6. Timing is essential. Take blood 11-12 hours post injection. 7. Withdraw blood and inject into red-top blood vials [sterile no additive vials] and allow the blood to clot. 8. Centrifuge immediately after clotting for clear serum. Do not allow RBCs to lyse. 9. Remove serum and place into sterile bottles. 10. Aliquot and store in freezer. Bottles of serum can be stored for up to five years. 11. Cryo-precipitates may form after refrigeration. Mixing causes clouding. This is not harmful. 13. Follow up with FFP protocol for puppies
Treatment of Newborn Puppies Using Fresh-Frozen Plasma during the First 10 Days of Life
Bring serum to room temperature as fluid should never be administered cool into puppies body as this can cause puppies to become chilled. Dose puppy before feeding time so any access fluid can be swallowed and digested.
The recommended dosage of 3 to 5 cc (ml) per pound can be given to each puppy orally in the first 24-36 hours.
Give to each pup orally, several drops at a time, every two hours up to three cc per one pound of body weight over the first 24-36 hours following whelp. Using a new eye dropper for administrating has been recommended by owners.
Beyond 36 hours of life the plasma must be given intraperitonealy or intravenously
Usually the pups only need to be treated once. However, if they appear to be fading, the dose can be repeated in 5-7 days later if necessary, but it must be IP or IV.
Do not give more than 10cc at one time.
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